In the Roman Catholic Church, the readings are Isaiah 49:1-6; Psalm 71:1-6, 71:15, 71:17; 1 Corinthians 1:18-31; and John 13:21-33, 13:36-38.
Holy Tuesday is notable for being the day on which the chrism mass is celebrated in each diocese. Usually held in the diocese's cathedral, in this mass the holy oils are blessed by the bishop, consisting of the chrism, oil of the sick, and oil of catechumens. The oil of the catechumens and chrism are to be used on the coming Holy Saturday at the Easter Vigil, for the baptism and confirmation of those entering the church.
In the Eastern Orthodox Church, and those Eastern Catholic Churches that follow the Byzantine Rite, this day is referred to as Great and Holy Tuesday, or Great Tuesday. On this day the Church commemorates the Parable of the Ten Virgins (Matthew 25:1-13), which forms one of the themes of the first three days of Holy Week, with its teaching about vigilance, and Christ as the Bridegroom. The bridal chamber is used as a symbol not only of the Tomb of Christ, but also of the blessed state of the saved on the Day of Judgement. The theme of the Parable of the Talents (Matthew 25:14-30) is also developed in the hymns of this day.
The day begins liturgically with Vespers on the afternoon of Great Monday, repeating some of the same stichera (hymns) from the night before. At Great Compline a triode (Canon composed of three Odes), written by St. Andrew of Crete is chanted.
The Matins service for Monday through Wednesday of Holy Week is known as the Bridegroom Service or Bridegroom Prayer, because of their theme of Christ as the Bridegroom of the Church, a theme movingly expressed in the troparion that is solemnly chanted during them. On these days, an icon of "Christ the Bridegroom" is placed on an analogion in the center of the temple, portraying Jesus wearing the purple robe of mockery and crowned with a crown of thorns (see Instruments of the Passion). These Matins services are often chanted the evening before so more of the faithful may attend. The Matins Gospel read on this day is from the Gospel of Matthew 22:15-23:39.
The four Gospels are divided up and read in their entirety at the Little Hours (Third Hour, Sixth Hour and Ninth Hour) during the course of the first three days of Holy Week, halting at John 13:31. There are various methods of dividing the Gospels, but the following is the most common practice:
Holy and Great Tuesday
- Third Hour—The second half of Mark
- Sixth Hour—The first third of Luke
- Ninth Hour—The second third of Luke
At the Liturgy of the Presanctified Gifts, some of the stichera from the previous night's Matins (Lauds and the Aposticha) are repeated at Lord, I have cried (see Vespers). There are two Old Testament readings: Exodus 2:5-10 and Job 1:13-22. There is no Epistle reading, but there is a Gospel reading from Matthew 24:36-26:2.